Aim: To verify whether there is a difference in the value of pre-season biomechanical and functional tests scored by injured and uninjured players. Material and methods: 46 female players of the football academy took part in the pre-season biomechanical and functional evaluation including isokinetic tests, functional tests, fundamental movement screen tests and postural stability tests (Biodex, FMS, Optojump, Delos), and then completed the online questionnaire reporting their injuries in the last round of the season. Results: Out of 46 participants, 18 participants (19 injuries) suffered injuries in the area of the lower limbs. There was a difference between the groups (non-injured vs. injured) in the results of anthropometric measurements: age (13.0 vs. 25.0, p=0.001), height (159 cm vs. 167 cm, p=0.018), weight (48.5 kg vs. 60 kg, p<.001), as well as in the results of BMI (18.9 vs 21.5, p<.001), 3SLHT test for left leg (2.6 vs 2.8, p=0.044) and right leg (2.5 vs 2.95, p=0.001), FMS (20 vs 18, p=0.026), flexion power/kg (0.8 vs 0.9, p=0.039) and for the maximum time without contact test on the DELOS platform for the left leg (12.6 vs 16.6, p=0.022). Conclusions: There is a difference in the selected biomechanical and functional outcomes between the group of injured and uninjured players. The footballers who scored higher values in jump tests and FMS reported injury occurrence more often during the football season. Additionally, injured players were older, higher, weightier and had higher BMI when compared to non-injured players.